Table 1.
Media supplement | Number of nucleated cell from marrow | Total number of BMSCs harvested | Days in culture | CFE per 1 × 105 cells | |
---|---|---|---|---|---|
BM01 | 10% HPGF-C18 | 10.0 × 106 | 1.20 × 105 | 14 | 2 |
20% FBS | 10.0 × 106 | 1.14 × 106 | 14 | 1 | |
BM03 | 10% HPGF-C18 | 6.10 × 106 | 3.00 × 105 | 12 | 4a |
20% FBS | 6.10 × 106 | 4.20 × 105 | 12 | 3 | |
BM04 | 10% HPGF-C18 | 55.2 × 106 | 7.8 × 105 | 13 | 5a |
20% FBS | 55.2 × 106 | 5.94 × 106 | 13 | 4 | |
BM06b | 10% HPGF-C18 | 5.8 × 106 | 2.2 × 106 | 12 | 9a |
20% FBS | 5.8 × 106 | 3.78 × 106 | 12 | 11 | |
BM07b | 10% HPGF-C18 | 21.7 × 106 | 2.4 × 106 | 12 | 30a |
20% FBS | 21.7 × 106 | 1.14 × 106 | 12 | 25 |
aColonies with atypical features
bParticles were allowed to settle for the preparation of HPGF-C18