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. 2018 Mar 14;13:6. doi: 10.1186/s12263-018-0595-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Confocal immunofluorescent pictures (× 10) of rat livers and protein expression analysis. a Single channel pictures of stained nucleus (blue), actin filament (yellow), p16 INK4a antibody Alexa Flour 647 conjugated (red), and p21 Cip1 antibody Alexa Flour 488 conjugated (green). b Merged pictures (p16 INK4a/actin/nucleus and p21 Cip1/actin/nucleus, respectively) are selected and presented respectively to visualize protein quantification and localization. c p16 (INK4a) and p21 (Cip1) quantified protein level in C and HF rats as determined by immunofluorescence signal intensity ratio to nucleus signal intensity (n = 5). Data are presented as protein immunofluorescent sphere area to nucleus sphere area. Values are mean ± SEM, P < 0.05*