(A) qPCR for ERα and PGR expression in the MCF-7-miR-335 cell line vs. –vector. (B) Crystal Violet assay for cellular survival in the MCF-7-miR-335 cell line vs. -vector following the pretreatment treatment of E2 (1 nM) for 30 minutes followed by treatment with the endocrine therapies ICI 182,780 (100 nM) and 4-OH tamoxifen (100 nM). Normalization is to vehicle treated cell line designated as 100. (C) qPCR for pre-mir-335, miR-335-5p, and miR-335-3p in the MCF-7-parental, 4-OH tamoxifen (TAMR) resistant and ICI 182,780 (F) resistant cell lines. Normalization was to MCF-7-parental and U6. Error bars represent SEM. * p < 0.05, ** p < 0.01.