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. Author manuscript; available in PMC: 2018 Jul 25.
Published in final edited form as: Nature. 2018 Jan 17;553(7689):496–500. doi: 10.1038/nature25442

Extended Data Figure 3. Imaging of LIPSTIC labeling.

Extended Data Figure 3

a, Experimental set up for live imaging of LIPSTIC labeling. Cd40−/− B cells and Cd40lg−/Y CD4+ T cells were transduced with G5-CD40 (Tomato reporter) or CD40L-SrtA (GFP reporter), respectively. CD40L-SrtA+ T cells were loaded with AlexaFluor647-SELPETGG, mixed with G5-CD40+ B cells on intercellular adhesion molecule (ICAM)-coated chambers to allow interactions, and immediately imaged. b, Time series showing transfer of AlexaFluor647-SELPETGG (white) from CD40L-SrtA+ T cells (green) to G5-CD40+ B cells (red) upon interaction. Data are representative of two independent experiments.