Extended Data Figure 4. Generation of Cd40^G5 and Cd40lg^SrtA gene-targeted mice.

a, b, Schematic representation and CRISPR/Cas9 genome editing strategy for the Cd40^G5 (a) and Cd40lg^SrtA (b) alleles. c, Restriction fragment length polymorphism (RFLP) analysis of Cd40^G5/+ mice. c, PCR products generated using primers surrunding the G5 insertion site were digetsted with KpnI and analyzed by electrophoresis on an agarose gel. Data are representative of at least two experiments. d, Southern blot analysis of Cd40lg^SrtA/+ mouse. Genomic DNA was extracted, digested with XbaI, and transferred onto a nitrocellulose membrane after electrophoresis on an agarose gel. Genomic DNA fragments were detected using a probe annealing between exons 4 and 5.