Skip to main content
. 2001 Sep 4;98(19):10716–10721. doi: 10.1073/pnas.191362598

Figure 2.

Figure 2

Methylcellulose hematopoietic colony-forming assays. (A) Production of CFCs from human ES cells. H1/S17 cells, H1.1/S17, H1/C166 cells, and differentiated H1/MEF cells were harvested after 14–20 days of culture, placed in methylcellulose-based media supplemented with hematopoietic growth factors, and scored for total hematopoietic colonies after 14 days. Results are mean ± SE of seven trials with H1/S17, four trials with H1.1/S17, four trials with H1/C166, and three trials with H1/MEF. Data are presented as colonies per 105 cells harvested from the differentiated H1 cultures. (B) Time course of H1/S17 cell differentiation into hematopoietic CFCs. H1 cells cocultured with S17 cells for the indicated number of days before colony assay. (C) Percent of erythroid (burst-forming unit-erythroid) and myeloid (CFU-GM, CFU-M, and CFU-G) colonies derived from H1/S17 cells harvested at day indicated. (D) CD34+ H1/S17 cells are enriched for CFCs. Unsorted H1/S17 cells and H1/S17 cells sorted for CD34+ cells and CD34− cells by magnetic column were placed in hematopoietic colony assay. These results are mean ± SE of three separate trials.