Fig. 1.
IDL7 is localized to the apoplast. C-terminal GFP tags were cloned in-frame with IDL7, expressed in N. benthamiana epidermal cells under control of the 35S promoter, and analyzed using confocal laser scanning microscopy. The GFP control was localized in the cytosol and the nucleus (A), confirmed by plasmolysis (1 M NaCl, 30 min) (B). (C) IDL7∆SP–GFP was localized in the cytosol, confirmed by plasmolysis (1 M NaCl, 30 min) (D). (E) IDL7FL–GFP was found clogged in vesicular compartments, confirmed by plasmolysis (1 M NaCl, 30 min) (F). (G) IDL7∆C–GFP was localized in the apoplastic space, confirmed by plasmolysis (1 M NaCl, 30 min) (H). Arrows indicate plasmolysed plasma membrane. Scale bar=20 ìm.