Fig. 7.
IDL6 and IDL7 are negative modulators of stress-induced ROS signalling. (A) Measurements of electrolytic leakage after treatments with the ROS-generating enzyme assay xanthine/xanthine oxidase (0.05 U) 2, 6, 12, and 24 h after treatment (n=4). Statistical differences for all experiments (Student’s t-test: *P-value <0.05, **P-value <0.01, ns indicates P-value >0.05) between the wild-type Col-0 and mutants are indicated. Error bars indicate SDs. (B, C) Modulation of flg22-induced oxidative burst by IDL7 peptide. Arabidopsis Col-0 wild-type (B) or idl7 (C) leaf disks were exposed to flg22, IDL7-EPIP, IDL7-PIP, IDL7-PIPo, or MOCKIDL7 peptides (100 nM), either alone or in combination (flg22+IDL7, flg22+IDL7-PIP, flg22+IDL7-PIPo, and flg22+MOCKIDL7). Water was added as a control. ROS production measured as luminescence was monitored over time as relative light units (RLU). Error bars indicate the SE of n=12 replicates.