Skip to main content
. 2017 May 24;68(13):3573–3584. doi: 10.1093/jxb/erx170

Fig. 1.

Fig. 1.

CIPK6 is a negative regulator of plant defense. (A) Expression of CIPK6 in the wild type (Col-0) and different Arabidopsis lines used in this study as determined by RT–PCR. A lane with RNA from Col-0 without using reverse transcriptase (–RT) is shown to rule out genomic DNA contamination. Actin2 transcript was used as the control. (B) Bacterial growth content assay. Pst DC3000 (empty plasmid vector, EV) (OD600=0.0005) was manually infiltrated into leaves of the various Arabidopsis plants mentioned. Bacterial growth was assessed at 0 and 3 days post-infection (dpi) and was expressed as log of colony-forming unit per miligram of fresh weight (log cfu/mg fw). The asterisks indicate a significant difference following two-way ANOVA (α=0.05). (C–F) Pst DC3000 expressing AvrRpm1, AvrB, AvrRps4, or AvrRpt2 from plasmid vectors were manually infiltrated (OD600=0.001) into the rosette leaves of Col-0, cipk6, and CIPK6OX plant lines. Bacterial growth was assessed at 0 and 3 dpi and is presented similarly to as described above.