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Expression of the defense marker gene PR1 was enhanced in the absence of CIPK6. (A–E) Time course of PR1 expression assessed by qRT–PCR in Col-0, cipk6, CIPK6 overexpression, and cipk6/PCIPK6::CIPK6 plant lines in response to Pst DC3000 (EV) and to Pst DC3000 (AvrRps4/AvrRpm1/AvrB/AvrRpt2) infiltration. Actin 2 and Tubulin 4 were used as internal controls.
