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. 2017 May 24;68(13):3573–3584. doi: 10.1093/jxb/erx170

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — CIPK6 is a negative regulator of PTI- and ETI-triggered ROS generation. (A) Leaves of the Arabidopsis lines mentioned were manually infiltrated with Pst DC3000 (EV) (OD₆₀₀=0.001), Pst DC3000 (ΔhrcC), Pst DC3000 (AvrRps4), or Pst DC3000 (AvrRpm1) (OD₆₀₀=0.02). Hydrogen peroxide accumulation was detected by 3',3'-diaminobenzidine (DAB) staining at 5 hpi. (B–E) Time-course of ROS production in response to Pst DC3000 (EV), Pst DC3000 (ΔhrcC), Pst DC3000 (AvrRps4), and Pst DC3000 (AvrRpm1). ROS were measured for 250 min. The values presented are the mean of at least six biological replicates, each with four technical replicates. (This figure is available in colour at JXB online.)