Fig. 7.

Expression analyses of PTI marker genes in response to various elicitors in the absence of CIPK6. qRT–PCR analysis of FRK1, CYP81F2, WRKY22, WAK2, WRKY29, and FOX expression in Col-0 and cipk6 plants. Plants (8 d old, 21–23 °C) were treated with water, flg22 (1 µM), elf18 (1 µM), chitin (50 µg ml^–1), or peptidoglycan (PGN; 50 µg ml^–1). The samples were collected after 4 h of treatment for qRT–PCR analysis. The error bars in the qRT–PCR analysis indicate the SD. Expression of Actin 2 and Tubulin 4 was used as internal controls. At least three biological replicates for each sample were used for qRT–PCR analysis and at least two technical replicates were analyzed for each biological replicate.