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. 2017 Oct 14;216(12):1579–1591. doi: 10.1093/infdis/jix547

Figure 4.

Figure 4.

Depletion of regulatory T cells (Tregs) reactivates human immunodeficiency virus type 1 (HIV-1) replication in T cells but not in myeloid cells in humanized mice. Humanized mice were treated as described in Figure 1A and euthanized 12 weeks after infection. A, Representative histogram shows the percentage of HIVJRCSF gag p24+ cells in CD3+CD8− cells (CD4+ T cells), CD3−CD11c−CD4+CD123+ cells (plasmacytoid dendritic cells [pDCs]), CD3−CD4+CD14+ cells (monocytes/macrophages [mono/mac]), or CD3−CD14−CD4+CD11c+ cells (myeloid dendritic cells [mDCs]) in spleen and bone marrow. B, Summarized frequencies of HIVJRCSF gag p24+ cells in CD4+ T cells, pDCs, mono/mac, or mDCs in spleen and bone marrow specimens. C, HIVJRCSF p24 in spleen specimens was detected by immunofluorescence staining with anti-human CD3 (green), anti-HIV-1 p24 (red), and DAPI (blue). Data are representative results for merged (top) and individual (bottom) stains. Data are pooled from 2 experiments. Error bars display standard deviations. cART, combination antiretroviral therapy; DD, denileukin diftitox; HBSS, Hank’s balanced salt solution; PBS, phosphate-buffered saline. *P < .05.