. 2017 Jun 21;68(13):3351–3363. doi: 10.1093/jxb/erx196

Table 1.

HPLC analysis of B₁ vitamers in leaves and storage roots of selected cassava accessions

A
Leaves	TPP		TMP		Thiamin		Total vitamin B₁ (ng mg FW^–1)
	ng mg FW^–1	% total vitamin B₁	ng mg FW^–1	% total vitamin B₁	ng mg FW^–1	% total vitamin B₁	Total vitamin B₁ (ng mg FW^–1)
ARG 13	0.07 ± 0.09^a	55 ± 15	0.04 ± 0.02^a	45 ± 15	nd	nd	0.11 ± 0.11^a
BRA 132	0.12 ± 0.02^a	63 ± 8	0.07 ± 0.03^a	37 ± 8	nd	nd	0.19 ± 0.04^a
KBH 2006/18	0.10 ± 0.06^a	44 ± 19	0.13 ± 0.04^ab	55 ± 19	nd	nd	0.21 ± 0.06^ab
TAI 3	0.14 ± 0.11^a	55 ± 27	0.09 ± 0.05^a	45 ± 27	nd	nd	0.24 ± 0.07^ab
COL 1505	0.19 ± 0.13^a	65 ± 6	0.11 ± 0.08^a	35 ± 6	nd	nd	0.30 ± 0.20^ab
Kibaha	0.15 ± 0.03^a	42 ± 13	0.23 ± 0.09^ab	58 ± 13	nd	nd	0.37 ± 0.10^ab
cv. 60444	0.27 ± 0.09^a	66 ± 21	0.14 ± 0.10^ab	34 ± 21	nd	nd	0.41 ± 0.07^ab
GUA 79	0.26 ± 0.19^a	40 ± 8	0.32 ± 0.18^b	57 ± 9	0.02 ± 0.02	3 ± 3	0.60 ± 0.39^b

B
Storage roots	TPP		TMP		Thiamin		Total vitamin B₁ (ng mg FW^–1)
Storage roots	ng mg FW^–1	% total vitamin B₁	ng mg FW^–1	% total vitamin B₁	ng mg FW^–1	% total vitamin B₁	Total vitamin B₁ (ng mg FW^–1)
GUA 79	0.31 ± 0.14^a	94 ± 5	nd	nd	0.02 ± 0.03^a	6 ± 5	0.33 ± 0.16^a
KBH 2006/18	0.40 ± 0.11^ab	73 ± 9	0.00 ± 0.00^a	0 ± 1	0.14 ± 0.03^c	26 ± 8	0.54 ± 0.09^ab
cv. 60444	0.52 ± 0.14^abc	90 ± 1	0.00 ± 0.00^a	0 ± 0	0.06 ± 0.01^ab	10 ± 1	0.58 ± 0.16^ab
Kibaha	0.53 ± 0.08^abc	91 ± 5	0.01 ± 0.01^ab	1 ± 1	0.05 ± 0.03^a	8 ± 4	0.59 ± 0.10^ab
TAI 3	0.56 ± 0.04^abcd	86 ± 4	0.01 ± 0.00^ab	2 ± 1	0.08 ± 0.04^abc	12 ± 5	0.65 ± 0.06^bc
BRA 132	0.65 ± 0.11^bcd	95 ± 3	0.01 ± 0.01^ab	2 ± 1	0.02 ± 0.02^a	4 ± 3	0.69 ± 0.12^bc
COL 1505	0.72 ± 0.16^cd	83 ± 7	0.02 ± 0.01^b	2 ± 1	0.13 ± 0.07^bc	15 ± 7	0.87 ± 0.16^c
ARG 13	0.81 ± 0.09^d	90 ± 3	0.01 ± 0.01^ab	2 ± 1	0.07 ± 0.02^abc	8 ± 3	0.89 ± 0.07^c

B₁ vitamer distribution in (A) leaves and (B) storage roots of greenhouse-grown cassava plants. Total vitamin B₁ content corresponds to the TMP, TPP, and thiamin contents for each replicate. Percentage of total vitamin B₁ corresponds to the proportion of each vitamer relative to the total vitamin B₁ content. Data presented are the mean±SD of four biological replicates. Significant differences (P<0.05; Tukey’s multiple comparison test) are indicated by different letters. FW, fresh weight; nd, not detected.