Table 3.
Reactivity of Antigen Bands of Malaria Rapid Antigen Test for Triton and Sodium Dodecyl Sulfate Inactivated Samples
| whole blood sample dilutions | Patient 1 parasitemia 826800 trof/μL |
Patient 2 parasitemia 29400 trof/μL |
Patient 3 parasitemia 102 trof/μL |
Negative control | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | 0.1% SDS | 0.1% Triton | Control | 0.1% SDS | 0.1% Triton | Control | 0.1% SDS | 0.1% Triton | Control | 0.1% SDS | 0.1% Triton | |
| Undiluted | 3+/2+ | 3+/1+ | 3+/2+ | 3+/2+ | 3+/2+ | 3+/2+ | ± / ± | ± / ± | ± / ± | -/- | -/- | -/- |
| 1:2 | 3+/1+ | 3+/1+ | 3+/1+ | 3+/2+ | 3+/2+ | 3+/2+ | ± / ± | ± / - | ± / ± | -/- | -/- | -/- |
| 1:4 | 3+/1+ | 3+/1+ | 3+/1+ | 3+/2+ | 3+/1+ | 3+/2+ | ± /± | ± / - | ± / - | -/- | -/- | -/- |
| 1:8 | 3+/1+ | 3+/± | 3+/1+ | 3+/1+ | 3+/1+ | 3+/2+ | ± / - | ± / - | ± / - | -/- | -/- | -/- |
| 1:16 | 3+/1+ | 3+/± | 3+/1+ | 3+/1+ | 3+/1+ | 3+/1+ | ± / - | -/- | ± / - | -/- | -/- | -/- |
Table shows the detection of HRP-2 antigen (Plasmodium falciparum–specific) and pLDH antigen (pan-Plasmodium), respectively, in undiluted and diluted whole blood samples from 3 P. falciparum–positive patients and 1 negative control donor using no inactivation procedure (control), 0.1% sodium dodecyl sulfate inactivation procedure, or 0.1% Triton inactivation procedure. Dilution of blood samples containing P. falciparum parasites was performed in uninfected control blood. Trof/μL is the number of P. falciparum trophozoites per microliter of whole blood. A negative test result is indicated by “-”, whereas positive test results are indicated by “±”, “1+”, “2+”, or “3+” (scale from weakly positive test to strongly positive test).
Abbreviaton: SDS, sodium dodecyl sulfate.