Figure 3.

MG1 infection of resting CD4⁺ T cells latently infected with human immunodeficiency virus type 1 (HIV-1) results in the elimination of replication-competent HIV-1. A, Experimental design for MG1 infection of an in vitro primary cell model of HIV-1 latency in resting CD4⁺ T cells. B, Integrated HIV-1 DNA level 48 hours after MG1 infection (n = 12) relative to that in MG1-uninfected controls, measured by quantitative polymerase chain reaction analysis. *P = .0086 by 1-way analysis of variance (ANOVA) on ranks, and P < .05 by the Dunn multiple comparison test. C, Copies of integrated HIV-1 DNA per 10⁶ cells 48 hours after MG1 infection (n = 6), measured by digital droplet PCR. *P = .0162 by 1-way ANOVA on ranks, and P < .05 by the Dunn multiple comparison test. D, Concentration of p24 antigen in supernatants at day 10 of a viral outgrowth assay (n = 9). *P = .0288 by 1-way ANOVA, and P < .05 by the Dunnett multiple comparison test. Data points in panel D are the average of duplicate technical replicates. Data represent mean values ± standard errors of the mean; n values represent separate biological replicates. Individual donors are defined by color in panels C and D. ELISA, enzyme-linked immunosorbent assay; GFP, green fluorescent protein; MOI, multiplicity of infection; PBMC, peripheral blood mononuclear cell.