Figure 2.

Substantial extramuscular transgene expression mediated by adeno-associated virus 8 (AAV8) and AAV9 vectors following intramuscular administration. A and B, Albino C57BL/6 mice (n = 4 per group) injected intramuscularly with 1 × 10¹¹ vector genomes of AAV8 (A) or AAV9 (B) vectors expressing firefly luciferase were imaged ventrally 1, 3, 7, 14, 21, 28, and 56 days after injection. C and D, The relative photon emission produced by luciferase from AAV8 (C) and AAV9 (D) was quantified at various time points from 1 to 56 days after injection, and the percentage of extramuscular transgene expression relative to the total transgene expression was graphed.