Figure 4.

Comparison of adeno-associated virus 6.2FF (AAV6.2FF)–mediated monoclonal antibody (mAb) cocktail (2G4 + 5D2) expression levels in mice following intravenous, single, or separate intramuscular injections. Serum mAb expression levels in C57BL/6 mice (n = 4) following AAV6.2FF-2G4/AAV-5D2 cocktail (4 × 10¹¹ vector genomes total) administration either combined and administered intramuscularly (1xIM) or intravenously (IV) or separated and administered in 2 intramuscular injections (2xIM) on either leg were evaluated by enzyme-linked immunosorbent assay (ELISA) on 7, 49, and 126 days after administration. No significant differences in mAb expression levels were detected by 2-way analysis of variance when comparing routes of administration or combined versus separate injections of AAV vectors. NS, not significant.