Figure 3.
Δ346 mutant is attenuated in growth and thermally unstable. Growth curves of wild type (WT) and mutant viruses in (A) C6/36 cells or (B) Vero cells. C, Thermal stability of viruses that were incubated at 4°C, 28°C, 37°C, or 40°C for 8 hours, and then titered on C6/36 cells. Relative infectivity was calculated as (focus forming units [FFU]/mLtest temperature/FFU/mL4°C) × 100 (mean ± standard error of the mean [SEM]). D, Infectivity of viruses when incubated for various timings at 37°C. Relative infectivity was calculated as (FFU/mLtime indicated/FFU/mLt=0h) × 100 (mean ± SEM). One-way analysis of variance followed by Dunnett’s multiple comparisons test was conducted on log-transformed data (*P < .05). Dotted lines represent limit of detection.