Fig. 5.
WOX11 is involved in controlling transcripts of metabolism-related genes. (A) Metabolism GOs as indicated are enriched in the differentially expressed genes. Values of the x-axis are −logP. (B) qRT-PCR validation of metabolism-related genes regulated by WOX11 in 7-day-old crown root tips (3–5 mm). The PCR signals were normalized with those of the ACTIN1 transcripts. Values are means±SD from three biological replicates. (C) ChIP-PCR analysis using anti-WOX11 antibody of 7-day-old wild type (HY), wox11, and OxWOX11 crown root tips (3-5mm). Upper panel, diagrams of the indicated loci. The transcribed regions (thick lines), transcription start site (TSS) and primer sets (P1 to P3) used in the ChIP experiments are indicated. Numbers indicate distance (kb) from TSS. Lower panel, ChIP assays with anti-WOX11 antibody. Data represent the mean of three biological replicates. Values are means±SD. Significant difference is indicated by asterisks (*P<0.05, **P<0.01, t-test).