Fig. 3.

Nitrate repression of flowering is dependent on the NPF6.3/NRT1.1 nitrate transceptor. Nitrate transporter NPF6.3/NRT1.1 mutants chl1-5 and chl1-9 (Col-0 background) were sown on vermiculite and watered once a week with N-free nutrient solution containing either 1 mM (light gray) or 3 mM (dark gray) KNO₃. At least 20 plants were used for each measurement. Data are means and SD of three independent biological replicates. Asterisks highlight significant differences as determined by Tukey’s Multiple Comparison test (P≤0.01).