Fig. 5.

FT is the flowering integrator that mediates nitrate-dependent repression of flowering time. ft-10 and soc1-2 mutants (Col-0 background) were sown on vermiculite and watered once a week with N-free nutrient solution containing either 1 mM (light gray) or 3 mM (dark gray) KNO₃. Nitrate-dependent flowering time and rosette leaf number were determined for 20–50 plants of each genotype (A). FT transcript levels of the wild-type (Col-0) and the smz/snz double-mutant (B). Plants were grown on Petri plates containing solid N-free nutrient medium supplemented with either 1 mM or 3 mM KNO₃. At the days indicated, plants were harvested, their RNA was extracted and subsequently used for qRT-PCR. The ADAPTOR PROTEIN-4 MU-ADAPTIN gene (At4g24550) was used as an internal reference. Three independent biological replicates of 15 plants were used. Data are means and SD. Asterisks highlight significant differences as determined by Tukey’s Multiple Comparison test (P≤0.05).