Figure 1.

Intramuscular enterovirus D68 (EV-D68) injection model for tracking paralytic disease over time. A, Dose response curve for mice given 10 (n = 21), 10² (n = 19), 10³ (n = 27), and 10⁴ (n = 14) 50% tissue culture infectious doses (TCID₅₀) of EV-D68 IL/14-18952 by intramuscular inoculation, as quantified by motor impairment score for mice surviving to the experimental end point 21 days after infection. B, Viral growth in muscle and spinal cord tissue specimens following intramuscular injection with 10³ TCID₅₀ of EV-D68 IL/14-18952 (n = 10 per time point). Error bars are standard errors of the mean.