Figure 4.

Fluoxetine has no effect on enterovirus D68 (EV-D68)–induced motor impairment and viral loads. A, Motor impairment scores for mice infected by intramuscular injection with 10³ 50% tissue culture infectious doses (TCID₅₀) of EV-D68 IL/14-18952 and then given fluoxetine by intraperitoneal injection for 7 days on days 0–6 after infection at 3 different concentrations (0.75 mg/kg [n = 7], 1.5 mg/kg [n = 9], or 3.0 mg/kg [n = 7]) versus vehicle control (n = 29). Treatment started the same day as viral infection, defined as day 0 after infection, and ended on day 6 after infection. Data for animals surviving to the end of the study were graphed. B and C, Mice were treated with 3.0 mg/kg fluoxetine (n = 11) for 7 days (days 0–6 after infection) or vehicle (n = 11) following intramuscular injection of 10³ TCID₅₀ EV-D68 IL/14-18952. On day 6 after infection, several hours after the final dose of fluoxetine, muscle (open squares) and spinal cord (filled circles) tissue specimens were collected for viral titer analysis. Error bars are standard errors of the mean. NS, not significant.