Fig. 4.
Expression analysis of subgroup III MATE genes using qRT-PCR. (A) Expression of four PtrMATE members in poplar roots under 500 μM Al3+ for 6 h and 24 h. (B) Relative quantities of four members in roots, stems, young leaves, and mature leaves are illustrated. Expression of PtrMATE1 in wild-type roots was arbitrarily fixed at one. The results are shown as the mean expression ± standard deviation (SD) of three independent experiments. Poplar ubiquitin (UBQ) expression was used as a control and gene-specific primers were used for qRT-PCR analysis of PtrMATE genes. Student’s t-test, *P<0.05, **P<0.01.