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. 2017 May 20;68(12):3215–3230. doi: 10.1093/jxb/erx162

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — CIPK9 and CIPK14 interact with CBL1 and ACA8 in BiFC assay. (A and B) GFP fluorescence associated with ACA8 in N. benthamiana leaves marks the cell periphery. The focus image (B) is a 5-fold magnification of the image in (A). The white arrow in the magnified image indicates the pattern of signal distribution in two neighbouring cells. (C–H) Biomolecular fluorescence complementation (BiFC) analyses of YN::CIPK9, YN::CIPK14, and YN::CIPK7 with, respectively, CBL1::YC (C, E, G) and ACA8::YC (D, F, H) in transiently transformed N. benthamiana leaves. CIPK9, CIPK14, and, to a lesser extent, CIPK7 show interaction with ACA8 as indicated by the reconstitution of the YFP fluorescence (reported as green colour) at the cell periphery that represents the plasma membrane. Results, collected 4 d after infiltration with A. tumefaciens, are from one experiment representative of at least five independent experiments. Scale bars=40 μm.