Fig. 6.

Cytosolic Ca²⁺ transients in response to wounding are altered in the aca8 single knock-out mutant. Leaves of wt or aca8 plants that constitutively express cytosolic YC3.6 were wounded (arrow). FRET changes were measured in a small area proximal to the wound site (~1 mm²). Traces (A) represent the raw FRET cp Venus/CFP ratio variations observed during the entire experiment and are the averages from the analysis of 23 independent wounding events performed on 23 independent leaves of 10 different plants for each genotype. (B) Statistical analysis of raw cpVenus/CFP ratios measured before challenging wt or aca8 leaves with wounding. (C) Statistical analysis of the normalized cpVenus/CFP ratio of the first and second peaks of the Ca²⁺ transients measured in wt or aca8 seedlings and reported as ΔR/R₀. Values are means ±SEM. Asterisks indicate statistically significant differences (**P<0.05) calculated using Student’s t-test.