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. 2018 Jan 5;69(3):603–617. doi: 10.1093/jxb/erx441

Fig. 7.

Fig. 7.

Subcellular localization of GmPAP1-like and root-associated APase activity in transgenic bean hairy roots overexpressing GmPAP1-like. (A) Bean hairy roots expressing 35S::GFP (top row) and 35S:: GmPAP1-like-GFP (bottom row) were observed by confocal laser scanning microscopy. Scale bars are 20 μm; (B) Root-associated APase activity of bean hairy roots transformed with the empty vector (CK1) or GmPAP1-like (OX1) detected by BCIP staining. Scale bars are 5 mm. (C) Root-associated APase activity of transgenic bean hairy roots. CK1, CK2, and CK3 represent three transgenic hairy root lines transformed with the empty vector; OX1, OX2, and OX3 indicate three transgenic hairy root lines with GmPAP1-like overexpression. Data are means of four biological replicates ±SE. Asterisks indicate significant differences between GmPAP1-like overexpression lines and CK lines: *P<0.05.