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Electrophoretic mobility shift assay (EMSA) of EjNAC3 binding to the EjCAD-like promoter. (A) The probe sequence used for EMSA. (B) Purified EjNAC3 protein and biotin-labeled DNA probe were mixed and analysed on 6% native polyacrylamide gels. The presence (+) or absence (–) of specific probes is indicated. The concentration of the cold probe was 100 nM (+) or 300 nM (++), while that of the biotinylated probe was 1 nM.
