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. 2017 Dec 22;69(3):385–397. doi: 10.1093/jxb/erx427

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© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig 4. — Tissue-specificity and subcellular localization of OsPIN2. (A) Tissue-specificity in the root tip. Immunostaining was performed with an anti-PIN2 antibody in root tips of the wild-type (WT) and lra1 (longitudinal sections). The boxed areas are magnified in the images to the right. Abbreviations: ep, epidermis; ex, exodermis; scl, sclerenchyma; co, cortex. Arrows indicate polar localization of OsPIN2. Scale bars =20 µm. (B) Subcellular localization of OsPIN2. The OsPIN2-GFP fusion protein driven by the OsPIN2 promoter and CHL1-mCherry (cell membrane marker) driven by the 35S promoter were transiently co-expressed in rice protoplasts. Fluorescent signals as observed by confocal microscopy are shown. Scale bars =5 µm.