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. 2017 Jun 29;68(16):4463–4477. doi: 10.1093/jxb/erx220

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Diurnal profiles of glutamine (Gln) and glutamate (Glu) in arabidopsis plants growing in natural or artificial light with a 12-h photoperiod. Arabidopsis thaliana Col-0 plants were grown in a naturally illuminated greenhouse around the vernal equinox in 2012 (orange circles) and in controlled environment chambers with a 12-h photoperiod and daily light integral (DLI) of 7 mol m⁻² d⁻¹. Artificial illumination was provided by white fluorescent tubes (blue symbols) or LEDs (grey symbols), with either a constant (squares) or sinusoidal (triangles) light profile. Rosettes were harvested from 4-week-old plants throughout a 24-h diurnal cycle for metabolite analysis. (A) Gln and (B) Glu were measured by HPLC, and the Gln:Glu ratio is shown in (C). Data are means ±SD (n=3 for LED conditions and n=4 for the others). ZT, zeitgeber time (hours after dawn).