Fig. 8.
Fractionation of B. napus root exudates. (A) Distribution of EHS induction activity after RP-HPLC fractionation of SPE concentrated B. napus root exudates. Half-diluted exudates and SPE extracts (1000-fold diluted) were used as positive controls, Coïc medium was used as negative control. HPLC-purified fractions were 100-fold diluted. All dilutions were performed in buffer solution (0.5 mM HEPES, 0.05% PPM). Means are values ±SE (n=3). Means annotated with asterisks are significantly different from the negative control (t-test, ***P<0.001). (B). UPLC-ESI(+)-MS/MS analysis of fraction 9 detected in the MRN channel of DHZ.