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. 2017 Dec 14;69(3):579–588. doi: 10.1093/jxb/erx419

Fig. 2.

Fig. 2.

Suppression of CAU1-mediated histone methylation in the ANAC055 promoter region under drought stress. (A) Diagram of the ANAC055 gene. The genomic regions A to G used in the ChIP assays are indicated. (B, C) ChIP assays with antibodies against H4R3sme2 (B) using Col-0 and cau1 plants, and GFP (C) using 35S:EYFP-CAU1/cau1 plants. TUB8 was used as an internal control in (C). Three independent experiments were performed, and values are means ±SE. *P<0.05 and **P<0.01. (D, E) ChIP assays with antibodies against H4R3sme2 within the C region (D) or GFP within the B region (E) of ANAC055. Plants were treated with 10% PEG-6000 for the period indicated. Three independent experiments were performed. TUB8 was used as an internal control in (D). Values are means ±SE. Different letters above each bar indicate significant differences (ANOVA tests).