Fig. 1.

The cytosolic Ca²⁺ elevation in response to flg22 addition is greater than that in response to Pep3 when extracellular Ca²⁺ is limited. Extracellular Ca²⁺ was lowered in these experiments by addition of 10 mM BAPTA (A) or EGTA (B). Pep3 or flg22 ligand (20 nM) was added following 30 min pretreatment of WT (Col-aeq) seedlings with BAPTA or EGTA. Results are presented as mean values of cytosolic Ca²⁺ ±SE (n=3).