Fig. 3.

IP3 signaling is involved in flg22-induced Ca²⁺ rise and defense gene upregulation. (A) Exogenous flg22-dependent elevation of cytosolic Ca²⁺ in WT (Col-aeq) and IP5-ptase-aeq seedlings. Ligand (1 µM flg22) was added at time ‘0’; results are presented as mean values of cytosolic Ca²⁺ concentration (replicate number in parentheses) ±SE shown at 0.5 min intervals. Results shown in this figure are compilations from numerous biological replicates. Recordings from individual seedlings indicate that the Ca²⁺ elevation is transitory (e.g. Supplementary Fig. S2). (B) IP3 is involved in flg22-dependent WRKY29 and WRKY33 expression. Seedlings were incubated with ligand flg22 (light bars) or water addition (dark bars) for 30 min prior to RNA extraction. Results shown are means±SE (n=4) of transcript levels normalized to the level in WT (Col-aeq) seedlings in the absence of flg22. Asterisks indicate significant difference (P<0.05) between WT (Col-aeq) and IP5-ptase here seedlings treated with flg22.