Fig. 5.

InsP (IP3 and/or IP6) signaling is involved in flg22-induced NO generation. Results are shown for experiments comparing NO generation in (A) Col-aeq and IP5-ptase here plants and (B) Col and ipk1 null mutant plants. Signals shown (as bars) are means (±SE) of four replicates prepared from leaves of different plants. Fluorescence of approximately three guard cell pairs in an epidermal peel was averaged for one replicate. The maximum fluorescence intensity that could be recorded from the peels was 256 fluorescence units per pixel. Significant differences (at P<0.05) between fluorescence recorded from the genotypes used for an experiment are indicated by an asterisk above a bar.