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. 2017 Sep 2;216(9):1164–1175. doi: 10.1093/infdis/jix462

Figure 2.

Figure 2.

Yellow fever virus (YFV) env RNA expression specifically regulates protein tyrosine phosphatase receptor epsilon (PTPRE) expression. YFV Y274 peptide coding sequence aligned with 2 sites on the 3ʹ-UTR of PTPRE (A). PTPRE expression in Jurkat cells expressing YFV env, YFV env with Y274F or Y274A substitutions, or the YFV env coding RNA with a frameshift to abolish protein translation (YFV-FS) (B). PTPRE expression was normalized using actin. The mutations introduced into the Y274 env RNA to generate amino acid mutations are underlined (B). To determine if YFV env RNA targets PTPRE 3ʹ-UTR sequences, HEK 293T cells were transfected with plasmid DNA encoding GFP with either the YFV PTPRE 3ʹUTR target site 1 and site 2 sequence or the HCV PTPRE 3ʹ-UTR site 1 and site 2 sequence. GFP expression in cells transfected with the GFP-PTPRE plasmids alone, or co-transfected with various concentrations of plasmid DNA encoding YFV native env, YFV env frameshift (with no protein translation; YFV-FS) or the Y274A mutant env were examined 72 h posttransfection (C). Data represent the averages (± standard error of the mean) of 3 independent transfection experiments. * P < .01; †P < .05. Abbreviations: FS, frameshift; GFP, green fluorescent protein; HCV, hepatitis C virus; MFI, mean fluorescent intensity; PTPRE, protein tyrosine phosphatase receptor epsilon; VC, vector control; YFV, yellow fever virus.