Fig. 5.

Expression and regulation of genes related to production of E-geraniol. (A) Changes in expression of CitERF71 during Newhall sweet orange ripening. (B) Changes in expression of CitTPS16 during Newhall sweet orange ripening. Gene expression was calculated relative to the fruit 45 d after full bloom (set as 1). Data represent the mean ±SEs of three biological replicates. The values of LSD represent the significant differences at P<0.05. (C) In vivo interaction between CitERF71 and the target promoter of CitTPS16. Samples were infiltrated into N. benthamiana leaves. Firefly luciferase (LUC) and renilla (REN) luciferase were assayed 3 d after infiltration. The ratio of LUC/REN to the empty vector plus promoter was used as a calibrator (set as 1). Data represent the mean ± SEs of four biological replicates. Asterisks indicate significant differences (**P<0.01). (D) Yeast one-hybrid analysis of CitERF71 binding to the CitTPS16 promoter. Interaction was determined on SD medium lacking leucine in the presence of AbA (–Leu+AbA²⁰⁰). AD-p53 and pAbAi-p53 were used as positive controls. AD-empty and pAbAi-CitTPS16 were used as negative controls.