. 2017 Nov 24;217(4):572–580. doi: 10.1093/infdis/jix566

Table 1.

The frequency of memory B cells (Bmem) expressing neutralizing antibodies (Abs) tracks with the neutralizing Ab titers in blood

M6						M7				M24			M25
Subject	mAb	mAb IC₅₀	Plasma IC₅₀	Subject	mAb	mAb IC₅₀	Plasma IC₅₀	Subject	mAb	mAb IC₅₀	Plasma IC₅₀	Subject	mAb	mAb IC₅₀	Plasma IC₅₀
11	HPV-16.77	>333 nM	7350	11	HPV-16.55	1.6 pM	414000	11	HPV-16.95	>333 nM	33500	11	HPV-16.128	>333 nM	74100
	HPV-16.78	>333 nM			HPV-16.56	6.2 nM			HPV-16.96	5.3 pM			HPV-16.129	30.2 pM
	HPV-16.79	>333 nM			HPV-16.57	0.2 pM			HPV-16.97	1.0 pM			HPV-16.130	6.8 pM
	HPV-16.80	39.1 pM			HPV-16.58	14.2 pM			HPV-16.98	>333 nM		12	HPV-16.131	15.9 pM	172000
	HPV-16.81	>333 nM			HPV-16.59	48.4 nM			HPV-16.99	8.5 pM			HPV-16.132	21.2 pM
13	HPV-16.83	>333 nM	8750		HPV-16.60	0.4 pM			HPV-16.166	30.7 pM			HPV-16.133	>333 nM
	HPV-16.84	6.2 pM			HPV-16.61	0.2 pM		12	HPV-16.100	36 pM	8070		HPV-16.134	>333 nM
	HPV-16.85	>333 nM			HPV-16.62	>333 nM		13	HPV-16.101	>333 nM	611		HPV-16.135	3.0 pM
	HPV-16.86	>333 nM			HPV-16.63	3.4 pM		14	HPV-16.102	>333 nM	4310		HPV-16.136	2.2 pM
	HPV-16.87	>333 nM			HPV-16.64	5.1 pM						13	HPV-16.137	7.0 pM	155000
14	HPV-16.82	8.1 pM	18500		HPV-16.65	>333 nM							HPV-16.138	23.4 pM
					HPV-16.66	38.1 pM							HPV-16.139	75.6 pM
					HPV-16.67	>333 nM							HPV-16.140	>333 nM
				12	HPV-16.68	6.3 nM	105000						HPV-16.141	1.1 pM
					HPV-16.69	1.7 pM							HPV-16.142	68.3 nM
					HPV-16.70	3.3 pM							HPV-16.143	0.7 pM
					HPV-16.71	6.7 pM							HPV-16.144	0.9 pM
					HPV-16.72	3.9 pM							HPV-16.145	6.8 pM
					HPV-16.74	0.7 pM							HPV-16.146	3.8 pM
				13	HPV-16.90	1.2 pM	10700						HPV-16.147	0.9 pM
				14	HPV-16.73	1.8 pM	122000						HPV-16.148	16.9 pM
					HPV-16.75	>333 nM							HPV-16.149	0.1 nM
													HPV-16.150	5.0 pM
													HPV-16.151	6.0 pM
													HPV-16.152	11.9 pM
												14	HPV-16.153	>333 nM	87900
													HPV-16.154	0.8 pM
													HPV-16.155	11.6 pM
													HPV-16.156	6.7 nM
													HPV-16.157	24.3 pM
													HPV-16.158	1.9 pM
													HPV16.159	1.0 pM
													HPV16.160	67.0 pM
													HPV16.161	8.1 pM
													HPV16.162	2.8 pM
													HPV16.163	2.9 pM
													HPV-16.164	0.4 pM

At each indicated time point, monoclonal antibodies (mAbs) cloned from human papillomavirus type 16 (HPV-16)–specific Bmem are shown alongside their neutralization potency, or the concentration at which they achieved half-maximal virus inhibition. No mAbs were successfully cloned from the few HPV-16⁺ Bmem isolated from subject 12 at month (M) 6. The levels of neutralizing anti–HPV-16 Abs in blood, or plasma dilution at which half-maximal virus inhibition (IC₅₀) occurred, are also shown for comparison. The frequency of neutralizing mAbs cloned from HPV-16⁺ Bmem at each time point was ~31% (M6), ~82% (M7), ~56% (M24), and ~86% (M25). Neutralization potencies for mAbs cloned from Bmem at M6W1 and M24W1 are shown in Supplementary Table 1.