Activation of TIE2 signaling reduces mycobacterial burden. A, Representative images of ectopic vascularization and vascular leak in Tg(kdrl:egfp) embryos infected with Mycobacterium marinum–cerulean and injected with dextran–Texas red. Scale bar represents 100 µm. B, Quantification of vascular leak in M. marinum–infected embryos treated with AKB-9785. C, Quantification of bacterial burden by fluorometry in M. marinum–infected embryos treated with AKB-9785. D, Quantification of the bacterial burden by a colony-forming units (CFU) recovery assay 2 weeks after M. marinum infection in adult zebrafish treated with AKB-9785 from 1 week after infection. Error bars represent standard deviations. Statistical testing was performed by unpaired Student t tests, with 3 independent biological replicates for each panel. Abbreviation: DMSO, dimethyl sulfoxide.