TABLE 1 .
Marginal association between variables of interest and overall microbial compositiona
| Variable | Bray-Curtis distance |
Unweighted UniFrac distance |
Weighted UniFrac distance |
|||
|---|---|---|---|---|---|---|
| R2 (%) | P value | R2 (%) | P value | R2 (%) | P value | |
| gDNA concn (1 ng/μl to 1.28 × 10−5 ng/μl) | 31.0 | <0.001 | 38.1 | <0.001 | 22.4 | <0.001 |
| 16S rRNA gene yield | 42.8 | <0.001 | 39.5 | <0.001 | 20.7 | <0.001 |
| Extraction method (Mo Bio vs Chemagic) | 0.8 | 0.57 | 0.7 | >0.79 | 0.6 | 0.78 |
| PCR cycling method (standard vs touchdown) | 1.1 | 0.37 | 1.5 | 0.23 | 3.7 | 0.03 |
| PCR volume (25 vs 50 μl) | 2.5 | 0.77 | 3.0 | 0.58 | 4.3 | 0.24 |
a
Associations were assessed by using permutational multivariate analysis of variance, based on Bray-Curtis, unweighted UniFrac, and weighted UniFrac distances. Percentages of variance explained (R2) and P values are shown. Abbreviation: gDNA, genomic DNA.