Fig. 2.

Aluminum accumulation in root tip. Roots of 5-day-old seedlings of three rice cultivars, i.e. Ka (Kasalath), Ko (Koshihikari), and R₁₃₂ (Rikuu-132), were treated for 24 h with 10 μM AlCl₃ + 0.2 mM CaCl₂ (pH 4.9) under light (LIGHT) or dark conditions in the presence of 1 mM mevalonate and 1 mM glucose (DARK[M,G]). More than six root tips were used for each cultivar, and two representative tips are shown. Aluminum accumulation was observed by hematoxylin staining; denser purple staining indicates greater Al accumulation. Scale bar: 1 mm.