Figure 2.

Sustained high level viral replication in ΔF508 mice after infection with Coxsackievirus. Wild-type (wt; black bars) and littermate ΔF508 mice (white bars) were infected with Coxsackievirus B3 (10² plaque-forming units [PFU]/mouse). A, Blood was harvested on days 3 (n = 3–5 animals per genotype), 4 (n = 9–12 animals per genotype), and 5 (n = 3–4 animals per genotype) postinfection for viremia measurements using plaque assay. B, Titers of replicating virus particles in organs harvested on day 5 postinfection were measured by plaque assay (n = 4–7 animals per genotype). C, Titers of replicating virus particles in organs harvested on day 7 postinfection were measured by plaque assay (n = 6–8 animals per genotype). A–C, Plaque assay results are presented as log₁₀(PFU/g wet tissue or mL blood) and represent means ± standard deviation *P < .05, **P < .01, ***P < .001 and n.s, nonsignificant, Mann–Whitney test.