Figure 4.
Mice carrying the ΔF508 mutation have a delayed antibody response to Coxsackievirus B3 (CVB3). A, Wt and ΔF508 mice were infected with 102 PFU CVB3/mouse. Virus-specific IgM and IgG antibodies were measured in serum samples harvested from infected animals on day 5 (n = 8–12 per genotype) and 7 (n = 3–5 per genotype) postinfection using a CVB3 virus like particles-specific enzyme-linked immunosorbent assay. B, C, Serum drawn on days 4 (n = 4–6 animals per genotype), 5 (n = 7–15 animals per genotype), and 7 (n = 3–8 animals per genotype) postinfection were evaluated for the presence of neutralizing antibodies, as described in Supplementary Material Methods. B, The percentage of antibody-positive wt (black bars) and ΔF508 (white bars) mice at the indicated days postinfection is shown. C, The percentage of antibody-positive wt mice (black bars, n = 8) and ΔF508 mice (white bars, n = 3) at indicated serum dilutions on day 7 postinfection. Data in A is presented as mean ± SD *P < .05, **P < .01. Mann–Whitney test (A) and Fisher exact test (B, C). Abbreviation: OD, optical density.