Table 1.
Selected Types of Amplified Molecular Tests for Detection of Mycoplasma genitalium
| Type of Test | Name of Test | Gene Target | Author | CE Mark |
|---|---|---|---|---|
| PCR | NA | MgPA | Deguchi et al [5] | No |
| PCR | NA | MgPA; 16S rRNA |
Jensen et al [6–8] | No |
| PCR | Duplex PCR | MgPA; 16S rRNA |
Hardick et al [9] | No |
| TMA | Aptima MG | 23S rRNA | Hologic 23]/Getman et al [24] | Yes |
| Multiplex PCR | STD Finder | MLPA | Muvunyi et al [25] | No |
| Multiplex PCR | Bio-Rad Dx | NA | Le Roy et al [26] | No |
| qPCR | Real-time PCR | pdhD | Müller et al [27] | No |
| Multiplex qPCR | AmpliSense | gyrB | Rumyantseva et al [28] | Yes |
| DNA microarray | Multiplex PCR-based microarray | 16S rRNA | Cao et al [29] | No |
| qPCR | Diagenode S-DiaMGTV | Mg219 gene | van Alphen et al [30] | Yes |
| Real-time PCR comparison |
TIB MOLBIOL/ Roche and Diagenode M. genitalium |
Mg219 and gap gene fragments | Le Roy et al [31] | No |
| Mulitplex qPCR PlexZyme | SpeeDX | 23S rRNA; detects resistant mutations |
Tabrizi et al [44] | Yes |
Abbreviations: MLPA, multiplex ligation-dependent probe amplification; NA, not applicable; PCR, polymerase chain reaction; qPCR, quantitative PCR; rRNA, ribosomal RNA; TMA, transcription-mediated amplification.