Fig. 1.

Spot test for the growth of C. reinhardtii strains in (A) high CO₂ (5% CO₂ in air) and (B) low CO₂ (0.01% CO₂ in air) pH 7.3. The strains include wild-type D66, the cia8 mutant, and three known CCM mutants cia6, cia5, and cia3 used as controls. The numbers to the left represent the initial OD₇₃₀ of 0.15 (~1.5 × 10⁶ cells) and two serial dilutions. (C) Growth of wild-type strain D66 and the cia8 mutant in liquid culture (MIN). Cultures were grown in Erlenmeyer flasks blowing in low CO₂ (0.01–0.025%). Values are expressed as the mean ± SE (n=4). (This figure is available in colour at JXB online.)