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RT–PCR analysis of D66 and cia8 mutant strains using poly(A) RNA from high and low CO2-grown cells as templates for RT–PCR. For the cia8 mutant, the primers shown in Supplementary Table S1 amplify a 1500 bp product from cDNA. GAPDH was used as a loading control and a 1000 bp product was amplified.
