Fig. 6.
High C/low N-cultivated hairy roots are affected in their oligofructan composition by 72 h abiotic stress exposures or phytohormone treatments. CiHRCs were first grown in standard medium (SM, 3% sucrose) for 1 week and then transferred to inulin induction medium (IMlowN, 6% sucrose) for 2 weeks, followed by 72 h of different stress or hormones treatments: (A) osmotic stress induced by 20% polyethylene glycol (PEG); (B) 100 mM NaCl; (C) 10 μM abscisic acid (ABA); (D) 100 μM indole-3-acetic acid (IAA); (E) 10 μM trans-zeatin riboside (cytokinin); and (F) 100 μM jasmonic aicd (JA). Carbohydrate measurements were performed via HPAEC-PAD with an ICS-5000 system and Carbpac PA1 column (Dionex). Representative fructan profiles of three independent replicates are shown. F, fructose; G, glucose; GF2, 1-kestotriose; GF3, 1,1-kestotetraose; GF4, 1,1,1-kestopentaose; S, sucrose.