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Activity of recombinant NDPK1 protein in the presence of small-molecule ligands measured using the pyruvate kinase–lactate dehydrogenase coupled assay. Measurements were performed with a fixed (0.004 M) TDP concentration and increasing amounts of small-molecule ligands. Percentage inhibition was calculated in relation to NDPK activity in the absence of the ligands. Data represent the average±SD, n=3. * indicates P<0.01 in a Student t-test.
