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. 2017 Sep 15;216(11):1452–1459. doi: 10.1093/infdis/jix488

Figure 1.

Figure 1.

CotE is essential for spore binding to mucus-producing HT29-MTX cells. A, Spores of wild-type (630Δerm, cotE+), cotE–, and cotEC– isogenic strains or cotE mutants carrying the pRPF185-CotE plasmid were added to mucus-producing HT29-MTX monolayers (multiplicity of infection of 100:1) aged 14 days. Non-adherent spores were removed by washing and the adherent spores were determined by plating on brain heart infusion broth supplemented with yeast extract, cysteine and sodium taurocholate agar. The experiment was independently repeated 3-times. Error bars represent standard deviations. **P ≤ .01, ***P ≤ .001 by the Welch unequal variance t test. B, Spores of 630Δerm (CotE+) were preincubated with anti-CotEC antibody or naive serum before adding to HT29-MTX cells. Non-adherent spores were removed by washing, and adherent spores were determined by plating on BHISS agar. Data for untreated spores and spore incubated with naive of anti-CotEC serum are shown. The experiment was independently repeated 3 times. Error bars represent standard deviations.